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Yeast Surface Display
129,69 € *
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In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. _

Anbieter: Dodax AT
Stand: 22.01.2020
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Yeast Display
29,90 € *
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High Quality Content by WIKIPEDIA articles! Yeast display (or yeast surface display) is a technique used in the field of protein engineering. The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. The technology was sold to Abbott Laboratories in 2001. A protein of interest is displayed as a fusion to the Aga2p protein on the surface of yeast. The Aga2p protein is naturally used by yeast to mediate cell-cell contacts during yeast cell mating. As such, display of a protein via Aga2p projects the protein away from the cell surface, minimizing potential interactions with other molecules on the yeast cell wall. The use of magnetic separation and flow cytometry in conjunction with a yeast display library is a highly effective method to isolate high affinity protein ligands against nearly any receptor through directed evolution.

Anbieter: Dodax AT
Stand: 22.01.2020
Zum Angebot
Yeast Display
29,00 € *
ggf. zzgl. Versand

High Quality Content by WIKIPEDIA articles! Yeast display (or yeast surface display) is a technique used in the field of protein engineering. The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. The technology was sold to Abbott Laboratories in 2001. A protein of interest is displayed as a fusion to the Aga2p protein on the surface of yeast. The Aga2p protein is naturally used by yeast to mediate cell-cell contacts during yeast cell mating. As such, display of a protein via Aga2p projects the protein away from the cell surface, minimizing potential interactions with other molecules on the yeast cell wall. The use of magnetic separation and flow cytometry in conjunction with a yeast display library is a highly effective method to isolate high affinity protein ligands against nearly any receptor through directed evolution.

Anbieter: Dodax
Stand: 22.01.2020
Zum Angebot
Yeast Cell Surface Engineering
112,13 € *
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This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.

Anbieter: Dodax
Stand: 22.01.2020
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Human Monoclonal Antibodies
152,93 € *
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This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma, production and purification of human monoclonal antibodies, humanization and optimization of monoclonal antibodies, rapid chimerization of monoclonal antibodies, epitope mapping via phage display from single gene libraries, recombinant antibodies made by combining phage and yeast display selections, production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells, and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Anbieter: Dodax
Stand: 22.01.2020
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Enhancing salt tolerance in corn using genetic ...
33,90 € *
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One corn (Zea maize L.) cultivar was used in this study (Giza 643), for its competence during the course of tissue culture, to improve its tolerance to salt stress via genetic transformation using glycerol-3-phosphate dehydrogenase (GPD1) gene isolated from Egyptian isolates of yeast (Saccharomyces cerevisiae) grown under salt and osmotic stress at concentrations 2M NaCl and 2M sorbitol, respectively. using simple and economic technique called Fluorescence differential display (FDD).

Anbieter: Dodax AT
Stand: 22.01.2020
Zum Angebot
Enhancing salt tolerance in corn using genetic ...
32,90 € *
ggf. zzgl. Versand

One corn (Zea maize L.) cultivar was used in this study (Giza 643), for its competence during the course of tissue culture, to improve its tolerance to salt stress via genetic transformation using glycerol-3-phosphate dehydrogenase (GPD1) gene isolated from Egyptian isolates of yeast (Saccharomyces cerevisiae) grown under salt and osmotic stress at concentrations 2M NaCl and 2M sorbitol, respectively. using simple and economic technique called Fluorescence differential display (FDD).

Anbieter: Dodax
Stand: 22.01.2020
Zum Angebot
Human Monoclonal Antibodies
152,93 € *
ggf. zzgl. Versand

This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma, production and purification of human monoclonal antibodies, humanization and optimization of monoclonal antibodies, rapid chimerization of monoclonal antibodies, epitope mapping via phage display from single gene libraries, recombinant antibodies made by combining phage and yeast display selections, production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells, and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.

Anbieter: Dodax AT
Stand: 22.01.2020
Zum Angebot
Yeast Cell Surface Engineering
116,21 € *
ggf. zzgl. Versand

This book provides a detailed and up-to-date overview of all aspects of yeast cell surface engineering, including fundamental principles, practical strategies for the construction of engineered yeasts, as well as medical and industrial applications. The technique makes it possible to add eukaryotic modifications to the surface-displayed proteins/peptides, which is of significant value in basic and applied research. Generally referred to as an arming (molecular display) technology, it allows yeast to be used as a whole-cell biocatalyst for a range of purposes, including bio-energy production, pollutant removal, recovery of rare metal ions, and preparation of functional cells, all of which are comprehensively covered in the book. Among the medical applications discussed are in vitro antibody preparation and the production of oral vaccines. In addition, it presents the latest advances in protein engineering and high-throughput screening for directed evolution of enzymes. The book enables graduate students and researchers to gain a deeper, comprehensive understanding of the technology, and offers further inspiration for researchers and industrial experts in this rapidly evolving field.

Anbieter: Dodax AT
Stand: 22.01.2020
Zum Angebot