One corn (Zea maize L.) cultivar was used in this study (Giza 643), for its competence during the course of tissue culture, to improve its tolerance to salt stress via genetic transformation using glycerol-3-phosphate dehydrogenase (GPD1) gene isolated from Egyptian isolates of yeast (Saccharomyces cerevisiae) grown under salt and osmotic stress at concentrations 2M NaCl and 2M sorbitol, respectively. using simple and economic technique called Fluorescence differential display (FDD).
Bacterial strains capable of decolourising azo dyes were screened and isolated. An optimal population mix, able to grow robustly in wastewater, stable and display high decolourisation efficiency was then developed. Several microbial consortia were formed and tested for their effectiveness with consortium C15 exhibited the greatest ability. Statistical optimisation of the media using Plackett-Burman and Box-Behnken designs, identified three critical nutritional factors, yeast extract, starch and corn steep solids that enhances the growth of the consortium and its decolourising capability. The consortium s potential usage in large scale treatments was tested on dye solutions and textile wastewater samples in a bioreactor. Overall, 80-100% colour removal was achieved within 48-72 hours. A stabilised inoculum, easily packaged and has long shelf life was then produced.
Selectins are cell adhesion molecules that initiaterecruitment of white blood cells to targeted sites onthe blood vessel wall. This process is necessary fornumerous immune functions, including inflammation,lymphocyte homing, and bone marrow replenishmentafter transplantation. Cell recruitment proceeds viaa multi-step process, with selectin-mediated rollingfollowed by integrin-mediated firm adhesion. Thisbook extends current knowledge of leukocyte adhesionby examining selectin-mediated adhesion in bothexperimental and computational studies.Selectin-ligand interactions were experimentallycharacterized using a yeast display system, in whichE-selectin-expressing yeast were perfused overligand-bearing surfaces. Structure-functionrelationships in selectin ligands were studied usingcell-free rolling experiments, in which ligand-coatedmicrospheres were perfused over L-selectin substrate.Finally, the roles of selectins and integrins in celladhesion were investigated using adhesive dynamic(AD) simulations. The model successfully reproducesin vivo data, and predicts synergistic functions ofthe two receptors in mediating leukocyte adhesion.
High Quality Content by WIKIPEDIA articles! Yeast display (or yeast surface display) is a technique used in the field of protein engineering. The yeast display technique was first published by the laboratory of Professor K. Dane Wittrup. The technology was sold to Abbott Laboratories in 2001. A protein of interest is displayed as a fusion to the Aga2p protein on the surface of yeast. The Aga2p protein is naturally used by yeast to mediate cell-cell contacts during yeast cell mating. As such, display of a protein via Aga2p projects the protein away from the cell surface, minimizing potential interactions with other molecules on the yeast cell wall. The use of magnetic separation and flow cytometry in conjunction with a yeast display library is a highly effective method to isolate high affinity protein ligands against nearly any receptor through directed evolution.
Infectious fungal diseases continue to take their toll in terms of human suffering and enormous economic losses. Invasive infections by opportunistic fungal pathogens are a major cause of morbidity and mortality in immuno-compromised individuals. At the same time, plant pathogenic fungi have devastating effects on crop production and human health. New strategies for antifungal control are required to meet the challenges posed by these agents, and such approaches can only be developed through the identification of novel biochemical and molecular targets. However, in contrast to bacterial pathogens, fungi display a wealth of "lifestyles" and modes of infection. This diversity makes it extremely difficult to identify individual, evolutionarily conserved virulence determinants and represents a major stumbling block in the search for common antifungal targets. In order to activate the infection programme, all fungal pathogens must undergo appropriate developmental transitions that involve cellular differentiation and the introduction of a new morphogenetic programme. How growth, cell cycle progression and morphogenesis are co-ordinately regulated during development has been an active area of research in fungal model systems such as budding and fission yeast. By contrast, we have only limited knowledge of how these developmental processes shape fungal pathogenicity, or of the role of the cell cycle and morphogenesis regulators as true virulence factors. This book combines state-of-the-art expertise from diverse pathogen model systems to update our current understanding of the regulation of fungal morphogenesis as a key determinant of pathogenicity in fungi.
In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering, yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. _
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma, production and purification of human monoclonal antibodies, humanization and optimization of monoclonal antibodies, rapid chimerization of monoclonal antibodies, epitope mapping via phage display from single gene libraries, recombinant antibodies made by combining phage and yeast display selections, production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells, and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Unique and thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.